lin28  (Cell Signaling Technology Inc)

Journal: Cell proliferation

Article Title: METTL16 and YTHDC1 Regulate Spermatogonial Differentiation via m6A.

doi: 10.1111/cpr.13782

Figure Lengend Snippet: FIGURE 2 | Mettl16 is essential for the differentiation of spermatogonia. (A) Gross morphology of representative testes from 8W control and age- matched Mettl16-sKO mice. (B) The testis/body weight ratio of 8W control and Mettl16-sKO mice. (C) H&E staining of adult control and Mettl16-sKO testes. (D) Immunofluorescent staining of c-KIT and LIN28A in adult control and Mettl16-sKO testes. Nucleus is stained using Hoechst33342 (blue). (E) Immunofluorescent staining of c-KIT in control and Mettl16-sKO testes at indicated days. (F) Quantification of c-KIT-positive cells in control and Mettl16-sKO testes at indicated days. (G) Immunofluorescent staining of γH2AX in 2-week-old control and Mettl16-sKO testes. (H) TUNEL assay of 2-week-old control and Mettl16-sKO testes. (I) Quantification of apoptotic cells in 2-week-old control and Mettl16-sKO testes. Data are presented as means ± SD (n = 3 for each group). p values were calculated with unpaired one-tailed Student's t-test (n.s., not significant, *p < 0.05, **p < 0.01, ****p < 0.0001). Scale bar = 50 μm.

Article Snippet: The following primary antibodies were used in this study: goat anti- PLZF (1:200, R&D, AF2944), goat anti- CD117/c- KIT (1:200, R&D, AF1356), rabbit anti- DDX4/MVH (1:200, Abcam, AB13840), goat anti- GFRA1 (1:200, R&D, AF560), rabbit antiSCP3 (1:200, Abcam, AB15093), rabbit anti- LIN28 (1:200, Proteintech, 11724- 1- AP) and rabbit anti- YTHDC1 (1:200, Abcam, AB220159).

Techniques: Control, Staining, TUNEL Assay, One-tailed Test

Journal: Cell proliferation

Article Title: METTL16 and YTHDC1 Regulate Spermatogonial Differentiation via m6A.

doi: 10.1111/cpr.13782

Figure Lengend Snippet: FIGURE 3 | YTHDC1 deletion in germ cells with Stra8-Cre leads to arrested differentiation of spermatogonia. (A) Gross morphology of representative testes from 16W control and age-matched Ythdc1-sKO mice. (B) The testis/body weight ratio of 16W control and Ythdc1-sKO mice. (C) H&E staining of adult control and Ythdc1-sKO testes. (D) Immunofluorescent staining of PLZF and DDX4 in adult control and Ythdc1-sKO testes. (E) Immunofluorescent staining of c-KIT and LIN28A in adult control and Ythdc1-sKO testes. Arrowheads indicate the c-KIT and LIN28A double- positive spermatogonia. (F) Immunofluorescent staining of c-KIT and LIN28A in PND8.5 control and Ythdc1-sKO testes. (G) Quantification of c-KIT- positive cells in PND8.5 control and Ythdc1-sKO testes. (H) TUNEL assay of PND8.5 control and Ythdc1-sKO testes. (I) Quantification of apoptotic cells in PND8.5 control and Ythdc1-sKO testes. Data are presented as means ± SD (n = 3 for each group). p values were calculated with unpaired one-tailed Student's t-test (**p < 0.01, ***p < 0.001). Scale bar = 50 μm.

Article Snippet: The following primary antibodies were used in this study: goat anti- PLZF (1:200, R&D, AF2944), goat anti- CD117/c- KIT (1:200, R&D, AF1356), rabbit anti- DDX4/MVH (1:200, Abcam, AB13840), goat anti- GFRA1 (1:200, R&D, AF560), rabbit antiSCP3 (1:200, Abcam, AB15093), rabbit anti- LIN28 (1:200, Proteintech, 11724- 1- AP) and rabbit anti- YTHDC1 (1:200, Abcam, AB220159).

Techniques: Control, Staining, TUNEL Assay, One-tailed Test